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. 2019 Jul 12;10:909. doi: 10.3389/fpls.2019.00909

FIGURE 5.

FIGURE 5

Induction of the Fe deficiency response by WCS417 in leaf Fe status shoot-to-root signaling mutant opt3-2. qRT-PCR analysis of FER1 gene expression in shoots (A) and MYB72 gene expression in roots (B) of 14-day-old Col-0 and opt3-2 plants growing on Fe-sufficient medium, 2 days after inoculation of the roots with WCS417 (OD600 = 0.01). (C) Shoot FW and representative pictures of Col-0 and opt3-2 plants, 7 days after inoculation of the roots with WCS417. (D) Total chlorophyll (a + b) content of Col-0 and opt3-2 plants, 7 days after inoculation with WCS417. Gene expression levels were normalized to that of the constitutively expressed reference gene PP2AA3 (At1g13320). Plotted are fold changes in gene expression levels relative to the average of non-inoculated Col-0 plants. Error bars represent standard errors of the mean. Different letters indicate statistically significant differences between treatments (one-way ANOVA followed by Tukey’s test; P < 0.05; n = 5–10). Scale bars in panel (C) represent 0.5 cm.