Figure 2.

Interaction of the MeCP2 Reader on the pri-miR-208b Promoter in Female Left Ventricle Is Independent of DNA Methylation and Involves RNA Interactions

(A) Exon 28 of Myh7 gene showing pri-miR-208b promoter with CpG Island (CGI). CGI sequence within pri-miR-208b promoter is conserved between mice and humans.

(B) Bisulfite conversion and DNA methylation show site-specific CG methylation in male and female left ventricle (LV).

(C) Procedure of ChIP coupled with ribonuclease degradation (+RNase A) to assess cardiac RNA-dependent interactions.

(D and E) (D) Specific binding of MeCP2 at pri-miR-208b promoter in male and female LV in the presence (+) or absence (−) of RNase A. (E) Intergenic promoter is shown as a control for methyl CpG-binding proteins.

(F) Representative immunoblots of MeCP2 protein expression in male and female LV. Bar graph represents mean values of LI-COR Odyssey quantification of protein blots normalized to histone H3.

**p < 0.01. n ≥ 4 mice per group. Data are represented as mean ± SEM. See also Figure S1.