Figure 3.

MeCP2 Forms an RNA/dsDNA Hybrid with Mhrt and pri-miR-208b

(A and B) Chromatin immunoprecipitation of (A) Rest and (B) Hdac2 binding at pri-miR-208b promoter in male and female LV in the presence (+) or absence (−) of RNase A.

(C) Enlarged view of the RNA/DNA hybrid region in predicted secondary structure of full-length Mhrt (also shown in Figure S3). The predicted Mhrt lncRNA (shown in orange)/DNA hybrid sequence at the pri-miR-208b promoter (shown in black). Twenty-five CG dinucleotides of Mhrt lncRNA are shown in red.

(D) In vitro reconstitution assay showing qPCR amplification of pri-miR-208b promoter after hybridization with Mhrt oligomer in the presence of RNase H or RNase V1.

(E) Amplification of pri-miR-208b promoter by qPCR after MeCP2 immunopurification from the Mhrt RNA-dsDNA hybrid.

*p < 0.05. ****p < 0.0001. Data are represented as mean ± SEM. See also Figures S2–S4.