Table 2. Effect of concentration of sucrose in preculture on the regrowth of cryopreserved in vitro shoot tips of blueberry by D cryo-plate method.
| Sucrose concentration | Regrowth (%±SE) |
|---|---|
| 0.3 M | 74.2±0.8 a |
| 0.5 M | 45.4±2.4 b |
| 0.7 M | 17.3±3.9 c |
Blueberry (Vaccinium ashei var. Tifblue) shoot tips were precultured for 1 day, LS treatment with 2 M glycerol+0.6 M sucrose for 30 min and desiccated for 1 h at 25°C. Regrowth: percent of shoot tips produced normal shoots after 1 month of plating. Approximately 10 shoot tips were tested for each of three replicates. Data followed by different letters are significantly different at p<0.05 (by Tukey’s test).