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. 2017 Mar 4;34(1):1–5. doi: 10.5511/plantbiotechnology.16.1231b

Table 2. Effect of concentration of sucrose in preculture on the regrowth of cryopreserved in vitro shoot tips of blueberry by D cryo-plate method.

Sucrose concentration Regrowth (%±SE)
0.3 M 74.2±0.8 a
0.5 M 45.4±2.4 b
0.7 M 17.3±3.9 c

Blueberry (Vaccinium ashei var. Tifblue) shoot tips were precultured for 1 day, LS treatment with 2 M glycerol+0.6 M sucrose for 30 min and desiccated for 1 h at 25°C. Regrowth: percent of shoot tips produced normal shoots after 1 month of plating. Approximately 10 shoot tips were tested for each of three replicates. Data followed by different letters are significantly different at p<0.05 (by Tukey’s test).