Table 3. Effect of preculture duration on the regrowth of cryopreserved blueberry in vitro shoot tips by D cryo-plate method.

Preculture duration	Regrowth (%±SE)
1 day	79.5±2.3 a
2 days	45.2±2.9 b
3 days	35.4±4.7 c

Blueberry (Vaccinium ashei var. Tifblue) shoot tips were precultured on 0.3 M sucrose, LS treatment with 2 M glycerol+0.6 M sucrose for 30 min and desiccated for 1 h at 25°C. Regrowth: percent of shoot tips produced normal shoots after 1 month of plating. Approximately 10 shoot tips were tested for each of three replicates. Data followed by different letters are significantly different at p<0.05 (by Tukey’s test).