Table 5. Regrowth of different cultivars of cryopreserved blueberry shoot tips derived from in vitro and current shoots by D cryo-plate method.
| Cultivars | Regrowth (%±SE) | t-test | |
|---|---|---|---|
| In vitro shoots | Current shoots | ||
| Rebiteye | |||
| Festival | 96.9±3.0 | 56.7±8.8 | * |
| Climax | 68.8±2.7 | 61.9±11.9 | NS |
| Highbush | |||
| Magnolia | 68.5±4.2 | 61.1±8.4 | NS |
| Sharpblue | 77.5±4.5 | 57.9±4.1 | * |
| Bluegold | 100.0 | 17.2±9.6 | ** |
| Brigitta | 90.2±5.3 | 62.7±6.5 | * |
| Weymouth | 46.7±2.8 | 37.1±2.4 | NS |
| Average | 78.4 | 50.7 | |
Shoot tips were precultured on 0.3 M sucrose for 1 day, LS treatment with 2 M glycerol+0.6 M sucrose for 30 min and desiccated for 1 h at 25°C. Regrowth: percent of shoot tips produced normal shoots after 4 weeks of plating. Approximately 10 shoot tips were tested for each of three replicates.