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. 2019 Jul 12;10:779. doi: 10.3389/fphar.2019.00779

Figure 3.

Figure 3

STS attenuated myocardial remodeling in mice after MI. (A) Representative heart photographs and the ratio of heart weight to body weight (HW/BW) are shown (scale bar = 2 mm). (B, C) Histological preparations stained with Masson’s trichrome, showing the effects of treatment on scar circumference (scale bar = 5 mm) and ventricular fibrosis (scale bar = 40 µm). (D) Hematoxylin and eosin-stained LV sections (scale bar = 40 µm). Cross-sectional area of myocardium was calculated in 10 randomly selected fields from each LV section, and five sections for each heart were measured. (E) Western blot analysis for atrial natriuretic peptide (ANP) by use of an anti-ANP antibody. Expression of tubulin served as a loading control. Representative blots from three independent experiments are shown. (F) Real-time quantitative PCR analyses for the expression of ANP messenger RNA. 18S expression was used as the internal control. # P < 0.05 versus sham; *P < 0.05 versus vehicle; & P < 0.05 versus STS. Veh, vehicle; STS, sodium tanshinone IIA sulfonate; Cq, chloroquine.