Fig. 4.

SOD3 is internalized and stored intracellularly. (A) HMDMs were added medium containing ovalbumin or cMyc-tagged SOD3 and incubated for 90 min. The medium was subsequently removed, the cells washed in the presence of heparin (50 U/ml) and cells subsequently lysed at the indicated time points. The recovered lysates were analyzed for the presence of ovalbumin and SOD3-cMyc by SDS-PAGE/Western blotting. The analysis was performed three times. This analysis shows that internalized SOD3-cMyc is maintained within the cell whereas ovalbumin is degraded.