Table 2.
Summary of the 6-propylthioruracil (PTU) concentration response replicates and assay performance across the test chemical plates tested in single-point and concentration-response.
| PTU Curve |
Z’ factor |
DMSO-MADb | PTU-MADc | ||||
|---|---|---|---|---|---|---|---|
| Platea | IC50 (μM) | SE | Hill slope | Mean | SD | (median; n=3) | (median; n=3) |
| TP | 5.92 | 0.69 | −0.99 | 0.7862 | 0.0589 | 2.909 | 5.553 |
| SP1 | 4.70 | 0.36 | −1.13 | 0.7544 | 0.0682 | 5.148 | 3.775 |
| SP2 | 5.35 | 0.43 | −0.95 | 0.7735 | 0.0957 | 3.467 | 5.201 |
| SP3 | 4.63 | 0.49 | −0.94 | 0.6825 | 0.0883 | 12.81 | 2.601 |
| SP4 | 4.47 | 0.45 | −0.89 | 0.7324 | 0.0121 | 5.971 | 1.883 |
| CR1 | 3.98 | 0.39 | −0.98 | 0.7056 | 0.0547 | 3.299 | 1.787 |
| CR2 | 6.43 | 1.04 | −1.16 | 0.5985 | 0.1467 | 6.843 | 5.520 |
a
TP was the plate used to test initial chemicals in concentration-response when establishing the assay. Plates SP1 – SP4 were plates of TCp1_v2 chemicals tested in single-point screen. Plates CR1 and CR2 were plates with TCp1_v2 chemicals tested in concentration-response mode.
b
DMSO-MAD is the platewise median absolution deviation calculated from all the DMSO wells in each of the three replicated assay plates.
c
PTU-MAD is the platewise positive control median absolute deviation calculated from all the 200 μM PTU wells in each of the three replicated assay plates.