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. Author manuscript; available in PMC: 2019 Jul 19.
Published in final edited form as: Nature. 2016 Oct 6;539(7627):48–53. doi: 10.1038/nature20122

Figure 2. Effect of McsB on the activity of ClpCP in vitro.

Figure 2

a, ClpCP-mediated degradation of β-casein in the presence of different effector proteins. Here and in the following, a quantification of the β-casein band is presented (original SDS–PAGE gels in Supplementary Fig. 2). Bottom panel shows the kinase activity of assayed McsB variants in an autoradiography plot. b, In contrast to active McsB, the inactive McsBE212A kinase cannot stimulate casein degradation by ClpCP. c, Effect of the YwlE arginine phosphatase on ClpCP activation by McsBA. The inactive YwlED118N mutant was used as a negative control.