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. Author manuscript; available in PMC: 2019 Jul 19.
Published in final edited form as: Nature. 2016 Oct 6;539(7627):48–53. doi: 10.1038/nature20122

Figure 4. ClpCP protease activity towards a pArg-containing substrate protein.

Figure 4

a, Preparation of the caseinpArg model substrate. SEC, size exclusion chromatography. b, Binding of caseinpArg (35 μM) to ClpC NTD (10 μM) at increasing amounts of pArgAA. c, Degradation of caseinpArg by ClpCP without adaptor proteins and the inhibitory effect of pArgAA on this activity. d, Pull-down experiment monitoring ClpCP complex formation in the presence of MecA, casein and caseinpArg. e, Preparation of substrate samples that contain increasing amounts of caseinpArg after prolonged incubation with McsBA. ClpCP degradation of resultant caseinpArg samples is directly correlated to the degree of substrate phosphorylation seen in pArg immunoblots (Extended Data Fig. 3e). AU, arbitrary units.