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. Author manuscript; available in PMC: 2019 Jul 19.
Published in final edited form as: Nature. 2016 Oct 6;539(7627):48–53. doi: 10.1038/nature20122

Extended Data Table 3. Expression and purification conditions.

Protein Expression Buffer A Buffer B
Bs ClpC 37°C, 3h 25 mM Tris pH 7.5, 300 mM NaCl 25 mM Tris pH 7.5, 300 mM NaCl
Bs ClpCDWB 37°C, 3h 25 mM Tris pH 8, 300 mM NaCl 25 mM Tris pH 7.5, 300 mM NaCl
Bs ClpCNTD 37°C, 2h 25 mM Tris pH 8.5, 150 mM NaCl, 1 mM TCEP 5 mM Tris pH 8.5, 50 mM NaCl, 1 mM TCEP
Ec ClpANTD 37°C, 2h 25 mM Tris pH 8.5, 150 mM NaCl, 1 mM TCEP 5 mM Tris pH 8.5, 50 mM NaCl, 1 mM TCEP
Bs ClpP 37°C, 3h 25 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol 25 mM Tris pH 7.5, 150 mM NaCl, 10% glycerol
Bs McsA 20°C, 5h 25 mM Tris pH 7.5, 300 mM NaCl, TCEP 1 mM 25 mM Tris pH 7.5, 100 mM NaCl, TCEP 1 mM
Bs McsB 18°C, O/N 25 mM Tris pH 7.5, 300 mM KCl 25 mM Tris pH 7.5, 50 mM KCl
Bs McsBE212A 18°C, O/N 25 mM Tris pH 7.5, 300 mM KCl 25 mM Tris pH 7.5, 50 mM KCl
Gs McsB 37°C, 3h 25 mM Hepes pH 7.5, 300 mM KCl 25 mM Hepes pH 7.5, 100 mM KCl
Gs McsBE122A 18°C, O/N 50 mM Tris pH 7.5, 50 mM NaCl 50 mM Tris pH 7.5, 50 mM NaCl
Bs MecA 25°C, 5h 25 mM Hepes pH 8, 300 mM KCl 25 mM Hepes pH 8, 300 mM KCl
Gs YwlE 37°C, 3h 25 mM Tris pH 7.5, 300 mM NaCl 25 mM Tris pH 7.5, 100 mM NaCl
Gs YwlED118N 37°C, 3h 25 mM Tris pH 7.5, 300 mM NaCl 25 mM Tris pH 7.5, 100 mM NaCl

Bs: Bacillus subtilis, Ec: Escherichia coli, Gs: Geobacillus stearothermophilus, O/N: overnight