(A) The total trimming activity for all 20 X-SHL8 substrates for each allotype combination were combined and grouped into CD8/TIL status. Overall trimming for each CD8/TIL group is represented as area under curve (AUC). (B) Table showing both the individual AUC for allotype pairs in each CD8/TIL group as well as the mean for each group. (C) Specificity of ERAP1 trimming activity from those found exclusively in CD8/TILhigh, CD8/TILmoderate or CD8/TILlow tumors towards X-SHL8 amino acids grouped based on physicochemical properties; basic, acidic, polar uncharged, aliphatic, aromatic and special. Data from (A-C) pooled from four independent experimental repeats ±SEM (***p = <0.001; **p = <0.01; *p = <0.05).