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. Author manuscript; available in PMC: 2020 Jan 1.
Published in final edited form as: Cancer Immunol Res. 2019 May 31;7(7):1202–1213. doi: 10.1158/2326-6066.CIR-18-0498

Figure 5. Generation of HPV E782-90 from natural precursors is impaired in CD8/TILlow ERAP1 allotype combinations.

Figure 5

E1KO 293T cells were transfected with ERAP1 allotype combinations from CD8/TILhigh, CD8/TILmoderate and CD8/TILlow or the non-functional active site mutant, E320A, together with minigenes encoding final LV9 or extended precursors D-LV9 or ED-LV9 and assessed for trimming activity by the activation of BE7A2Z. (A). Representative line graphs showing the trimming activity of selected ERAP1 allotype combinations from the three CD8/TIL groups. (B and C) The relative maximum LV9 response of ERAP1 allotype combinations from each group, where each symbol represents an individual ERAP1 allotype combination transfection. (B) Trimming activity towards a single amino acid extension (D-LV9) and (C) a double amino acid extension (ED-LV9; **** p = <0.0001; ** p = <0.01). (D and E) The relative maximum LV9 response of each individual ERAP1 allotype combination from CD8/TILhigh (black), CD8/TILmoderate (grey), and CD8/TILlow (white) towards D-LV9 (D) and ED-LV9 (E). Data pooled from three independent experimental repeats ±SEM.