Fig. 6.

The fermentation conditions have been optimized for the production of verticillin A and verticillin H. This was through both strain and media optimization via an OSMAC approach, where the droplet probe played an instrumental role. An average growth of strain MSX59553 yielded about 3 g of extract per flask in the lab. The data in orange are from three biological replicates (+/− SD), indicating about 10 mg of each compound per gram of extract (or 30 mg per flask). Initially, the cultures were generating about 1 mg of each compound per gram of extract (or 3 mg per flask).