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High-magnification NIR-IIb fluorescence imaging of vascular regeneration in the ischemic hindlimb. A, B, C. Fluorescence images of mouse hindlimb vasculature on days 0, 7 and 10 after induction of hindlimb ischemia. Shown are the vasculature in the ischemic limb and control (unoperated) limb. D. Quantification of microvessel formation in the ischemic limb during revascularization (n>4). Scale bar: 2 mm. Statistically significant difference in comparison with day 0 is shown as *P<0.05
