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. 2019 Jun 27;20(8):1093–1104. doi: 10.1111/mpp.12814

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© 2019 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of OsRboh‐mediated reactive oxygen species on RBSDV infection. (A) The effect of treatment with 100 μM diphenyliodide (DPI) on the expression of OsRboh. Each result represents not less than three biological repeats. * at the top of columns indicates significant difference from the mock‐treated control at P < 0.05. (B) Nitroblue tetrazolium (NBT) staining of rice seedlings treated with 100 μM DPI. (C) The effect of treatment with 100 μM DPI on the symptoms of RBSDV at 30 days post‐inoculation. (D) The effect of treatment with 100 μM DPI on the relative expression levels of RBSDV S6, S7 and S10 in RBSDV‐infected plants assessed by RT‐qPCR. Each treatment consisted of three biological replicates of at least 30 seedlings each. Different letters at the top of columns indicate significant differences at P < 0.05 (n ≥ 3) by Fisher's least significant difference tests.