Table 1.

Confirmation of RNA‐Seq gene expression data by semi‐quantitative RT‐PCR.

ID	Gene	Description	Expression level	Semi RT‐PCR WT/Δflp
XC_3001	hpa2	Lysozyme‐related protein Hpa2	2.97↓
XC_2324		c‐di‐GMP phosphodiesterase A	4.33↓
XC_3657	copB	Copper resistance protein B	2.58↓
XC_3129	pmrC	Inner membrane protein	3.59↓
XC_3597	hns	DNA‐binding protein	3.03↓
XC_3437	lptD	LPS‐assembly protein	2.29↓
XC_2004	avrXccC	Avirulence protein	8.31↓
XC_3694	ompW	Outer membrane protein	2.59↓
XC_2827	hpaR	MarR family transcriptional regulator	2.25↓
XC_0158		Xylosidase/arabinosidase	2.51↓
XC_2659	gcd	Quinoprotein glucose dehydrogenase	7.31↓
XC_1273	trkA	Voltage‐gated potassium channel	2.36↓
XC_1314	lptC	Lipopolysaccharide export system protein LptC	2.51↓
XC_3652	fabB	β‐ketoacyl‐[ACP] synthase	2.05↑
XC_0783	celS	Cellulase S	2.51↑
16S rRNA		Internal reference

16 genes of the transcriptome data were chosen to validate the integral accuracy via semi‐quantitative RT‐PCR. RNA, extracted from the cultures of Xanthomonas campestris pv. campestris wild‐type (8004) and Δflp, respectively, was reverted into cDNA, and then cDNA was used as the template in semi‐quantitative PCR. In this study, false discovery rate (FDR) ≤ 0.05 and absolute value of log₂ fold change ≥ 1 were used as the cut‐off values. The acquired results were accordant to the transcriptome data.↑, up‐regulated; ↓, down‐regulated.