Table 2.

Bacterial strains and plasmids.

Strain	Description	Source/reference
Pseudomonas syringae pv. phaseolicola (Pph) 1302A	Cause of halo blight disease in bean. Race 4	Taylor et al. (1996)
Pph 1448A	Race 6	Mansfield et al. (1994)
Pph RJ3	1302A variant PPHGI‐1‐	Jackson et al. (2000)
Pseudomonas syringae pv. tomato (Pto) DC3000	Pathogenic to tomato and Arabidopsis	Cuppels (1986)
Pph 1302A::NCR*	NCR insertion mutant	Pitman et al. (2005)
Pph 1302A::avrPphB *	avrPphB insertion mutant	Pitman et al. (2005)
Pph 1302A HR—P1, P2, P3, P4, P5, P6†	1302A HR‐causing passaged strains	This study
Pph 1302A D—P1, P2, P3, P4, P5, P6†	1302A Disease‐causing passaged strains	This study
Pph 1302A::oriVA‡	1302A variant	This study
Escherichia coli 39R861	Contains four plasmids used as size standards—154 kb, 66.2 kb, 37.6 kb and 7.2 kb	Threllfall et al. (1986)
Top10	Chemically competent cells	Invitrogen
TOPO cloning vector pCR2.1	KmR and ApR	Invitrogen

^* Pph 1302A variant with cloning vector pCR2.1 inserted into selected region in PPHGI‐1, Km^R.

^† Pph 1302A variants from each of six passages through Tendergreen (TG).

^‡ Pph variants with cloning vector pCR2.1 inserted into oriV in PPHGI‐1.

NCR, non‐coding region; Km^R and Ap^R, kanamycin and ampicillin resistant.