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. 2011 Mar 6;12(8):759–771. doi: 10.1111/j.1364-3703.2011.00709.x

Figure 2.

Figure 2

Characterization and gene expression analysis of Fusarium culmorum MCf 21 nit1 (21 nit) and seven independent TRI6 inverted repeat transgene (IRT) transformants (# 5, 14, 106, 111, 112, 114, 127). (A) Southern blot analysis using the ‘sense’TRI6 IRT fragment of vector pLRM13 to probe NcoI/HindIII‐digested genomic DNAs, resulting in a 1.7‐kb band for the endogenous TRI6 gene and a 1.4‐kb band in the case of TRI6 IRT integration. (B) Northern blot analysis of TRI6 expression using a 478‐bp‐long PCR‐amplified TRI6‐specific probe and control hybridization with the 18S probe. (C, D) Quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) on expression of the TRI6 gene (C) and the TRI5 gene (D). Normalization was achieved by comparing the expression of housekeeping genes β‐tubulin and 18S; broken line indicates MCf21 21 nit 1 expression level; all other values correspond to increased or decreased gene expression compared with the latter.