Table 2.

Fusarium culmorum MCf 21 nit1 and seven independent TRI6 inverted repeat transgene (IRT) co‐transformants. Section 1 contains data from Southern analysis, suggesting the presence of several IRT types which are defined here as follows: ideal IRT, a TRI6 IRT expressed by a single promoter and bound by a single terminator; promoter‐bound IRT, a terminator‐less TRI6 IRT driven by dual gpdA promoters; terminator‐bound IRT, a promoter‐less TRI6 IRT bound on either side by trpC terminators. As a result of the complexity of the integration patterns, it was not always possible to predict the exact type and number of IRT (i.e. 0–1, 1–2). Section 2 shows the production of deoxynivalenol (DON) and its acetylated derivatives (3‐AcDON and 15‐AcDON) and the severity of foot and crown rot in durum wheat.

Strain	1					2
	Ideal IRT	Promoter‐bound IRT	Terminator‐bound IRT	Various TRI6 fragments	Northern TRI6 smear	Total DON (ng/mL)*	Difference of means	Disease index (0–100)†	Difference of means
21 nit	0	0	0	0	No	898.1 ± 58.4		97.1 ± 1.9
5	0	0	1	?	No	890.6 ± 34.8	−7.5	97.9 ± 2.1	0.83
14	0–1	2	2	?	Yes	0**‡	−898.1	21.3 ± 4.9**	−75.75
106	1	1	2	0	Yes	0**	−898.1	33.3 ± 6.1**	−63.75
111	1	1	2	?	Yes	0**	−898.1	25.4 ± 3.2**	−71.67
112	0–1	0–1	0	1–2	Yes	96.1 ± 43.1**	−801.9	22.1 ± 7.6**	−75.00
114	1–2	0	0	0	Yes	0**	−898.1	40.4 ± 5.2**	−56.67
127	1–2	0	1–2	0	Yes	0**	−898.1	20.0 ± 5.3**	−77.08

^* Pooled data from two independent experiments (P= 0.171) are expressed as the sum of DON and its acetylated derivatives (ng/mL of culture broth) ± mean SE after 2 weeks of growth in Vogel's medium.

^† Pooled data from two independent experiments, carried out in 2008 and 2009 using wheat cultivars Prometeo and Solex, respectively (P= 0.626), are expressed as disease incidence (McKinney index 0–100; McKinney, 1923) ± mean SE 3 weeks after inoculation and sowing under glasshouse conditions.

^‡ Values in each column followed by two asterisks are significantly different from the MCf 21 nit1 control by Dunnett's test (P < 0.001).