Table 3.

Primers used throughout this study.

Application	Primer sequence	Amplicon size (bp)
Vector construction
TRI6senseF‐NcoI*	5′‐CCTCCATGGTCGTGTTGCGTCTCCCGATCC‐3′	570
TRI6senseR‐AscI	5′‐GCAGGCGCGCCACCCTGCTAAAGACCCTCAG‐3′
TRI6antisenseF‐NotI	5′‐CTTTGATGCGGCCGCGTCTCCCGATCCTG‐3′	570
TRI6antisenseR‐BamHI	5′‐GCATGGGATCCACCCTGCTAAAGACCCTCAG‐3′
Real‐time qRT‐PCR
TRI5forb	5′‐ACCCTCAATTCCTTCGTCGTATG‐3′	141
TRI5revb	5′‐CCCAAACCATCCAGTTCTCCATC‐3′
TRI6forb	5′‐TTATCGCCCTTCCCACCTTCAC‐3′	90
TRI6revb	5′‐TAAAGTCCCGTCCGCTCTCAAAG‐3′
18Sforb	5′‐TTGACCCGTTCGGCACCTTAC‐3′	75
18Srevb	5′‐AAGTTTCAGCCTTGCGACCATAC‐3′
tubfor	5′‐TTCCAAATCACCCACTCTC‐3′	104
tubrev	5′‐GAAAGTTGCCATCATACGG‐3′
Probes
TRI6probeF1	5′‐GACTTTGACAACTTCCCCACAT‐3′	478
TRI6probeR2	5′‐AGTGATCTCGCATGTTATCCAC‐3′
18S‐Forward	5′‐CCTTAACGAGGAACAATTGGAG‐3′	514
18S‐Reverse	5′‐CCCTAGTCGGCATAGTTTATGG‐3′
Restriction analysis of TRI4–TRI6–TRI5 cluster
H‐11 For4	5′‐GTGAACTTCGCGGGCGTTTACTC‐3′	5408
H‐11 Rev13	5′‐TCCGGCTTGAAGGTCGTCAAAAT‐3′

^* Restriction sites are given in italic.