Figure 4.

The citrus Ubc13 interacts with ubiquitin‐conjugating enzyme variant (Uev) and PthA proteins. (A) Sweet orange Ubc13 interacts with Uev and with PthAs 2 and 3. The disposition of the yeast co‐transformants in the plates is the same as shown in Fig. 1. (B) Western blots of glutathione transferase (GST) pulldown assays using GST–Ubc13 as bait and 6 × His–Uev and 6 × His–PthAs 2 and 4 as prey. According to the assay shown in (A), Ubc13 binds to Uev and PthA2, but not to PthA 4. Purified PthA proteins used as controls and the molecular sizes of the proteins are shown on the left. The corresponding sodium dodecylsulphate (SDS) gels are shown in Fig. S2 (see Supporting Information).