Skip to main content
. 2010 Jun 9;11(5):663–675. doi: 10.1111/j.1364-3703.2010.00636.x

Figure 5.

Figure 5

The repeat domain and leucine‐rich repeat (LRR) are important for protein–protein interactions. (A) Yeast two‐hybrid assays showing the interactions of the internal repeat domain of PthA2 (RD2) and the 5.5rep + CT2 construct with all citrus proteins. LRR interacted with α‐importin (α‐Imp) only. (B) Western blot of glutathione transferase (GST) pulldown assays showing that both RD2 and 5.5rep + CT2 constructs bind to citrus proteins (GST fusions). The C‐terminus of PthA2 (CT2), carrying LRR and the nuclear localization signal (NLS), binds to α‐Imp only. Purified PthA proteins used as controls and the molecular sizes of the proteins are shown on the left. The corresponding sodium dodecylsulphate (SDS) gels are shown in Fig. S2 (see Supporting Information).