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. 2008 Jun 28;9(5):633–643. doi: 10.1111/j.1364-3703.2008.00489.x

Figure 1.

Figure 1

WtsE‐induced cell death in a variety of non‐host plants. (A–D) Wild‐type or mutant P. stewartii strains carrying pRF205 were infiltrated at 2 × 108 CFU/mL into leaves of (A) N. benthamiana, (B) tobacco, (C) beet and (D) A. thaliana. (E,F) E. coli MC4100 (pCPP2156/pJH021) expressing the D. dadantii TTSS and WtsE or E. coli MC4100 (pCPP2156) without WtsE were infiltrated at 1 × 109 CFU/mL into leaves of (E) N. benthamiana and (F) tobacco. (G,H) Wild‐type wtsE or derivatives were transiently expressed by infiltrating 109 CFU/mL of Ag. tumefaciens C58C1 into leaves of N. benthamiana. (I) P. syringae pv. phaseolicola NPS3121 expressing WtsE or carrying a control vector were infiltrated at 1 × 108 CFU/mL into leaves of 4‐week‐old A. thaliana. Pictures were taken at 24 h (A–F) or 48 h (H–I) after infiltration or 24 h after spraying with dexamethsone at 48 h after infiltration (G) and are representative of three or more separate experiments.