Figure 1.

Immunoblot analysis of harpin from each strain of Pseudomonas syringae pv. tabaci (Pta). Each strain was grown in modified KB (2% proteose peptone No. 3, 1.6 nM MgSO₄·7H₂O, 8.6 mM K₂HPO₄, 1% glycerol) medium for 24 h and incubated in MMMF (50 mm potassium phosphate buffer, 7.6 mm (NH₄)₂SO₄, 1.7 mm MgCl₂, 1.7 mm NaCl, 10 mm mannitol, 10 mm fructose, pH 5.7) medium for 24 h. The bacterial culture was centrifuged, and the supernatant was concentrated 100‐fold. Harpin protein in the cell pellet and supernatant was detected as described previously by Taguchi et al. (2001). 1, Pta (wild‐type, WT); 2, Pta (phrpAZ); 3, PtaΔhrcC (phrpAZ); 4, Pta (phrpA); 5, Pta (phrpZ).