Table 3.

Effect of arginine auxotrophy on virulence and radial growth in vitro. Lesion areas of transformants and the wild‐type strain B05.10 on bean leaves were quantified using ImageJ, and are presented in arbitrary units (±standard error of the mean). The relative virulence of the transformants is shown as the proportion of the average lesion area of a transformant relative to the wild‐type (100%). N indicates the number of lesions measured. The relative colony diameter of the transformants in vitro is also indicated. n indicates the number of colonies measured.

Strain	Lesion area of mutant	Lesion area of wild‐type	Relative virulence of mutant	Relative colony diameter in vitro
Δbcass1	4300 ± 1100 (N= 17)	56700 ± 7800 (N= 17)	7.5%	0 (n= 3)
GFP1	15300 ± 900 (N= 5)	81200 ± 6300 (N= 5)	19%	77 ± 4% (n= 3)
GFP155	7500 ± 1000 (N= 7)	37700 ± 4300 (N= 7)	20%	83 ± 2% (n= 3)
GUS163	6700 ± 1500 (N= 5)	48000 ± 18000 (N= 5)	14%	85 ± 5% (n= 3)
LUC84	33500 ± 3900 (N= 5)	58700 ± 1600 (N= 5)	57%	87 ± 2% (n= 3)