Table 1.
Influence of the Δiad1/Δiad2 and Δnit1 deletions on the in vivo conversion of TAM or Trp to IAA.*
| Strain | TAM | Trp | ||
|---|---|---|---|---|
| IAA | ||||
| Mean ± SD (µm) | Mean ± SD (%) | Mean ± SD (µm) | Mean ± SD (%) | |
| FB1 | 1.58 ± 0.27 (6) | 100.0 ± 17.1 | 0.148 ± 0.025 (4) | 100.0 ± 16.9 |
| FB2 | 2.31 ± 0.60 (6) | 146.2 ± 38.0 | 0.140 ± 0.011 (4) | 94.6 ± 7.4 |
| FB1Δiad1 | 0.28 ± 0.06 (3) | 17.7 ± 3.8 | 0.050 ± 0.015 (4) | 33.8 ± 10.1 |
| FB2Δiad1 | 0.32 ± 0.10 (3) | 20.3 ± 6.3 | 0.039 ± 0.010 (4) | 26.4 ± 6.8 |
| FB1Δiad2 (GRN1) | 1.05 ± 0.32 (6) | 66.5 ± 20.3 | 0.130 ± 0.014 (4) | 87.8 ± 9.5 |
| FB2Δiad2 (GRN3) | 3.19 ± 0.31 (6) | 201.9 ± 19.6 | 0.130 ± 0.009 (4) | 87.8 ± 6.1 |
| FB1Δiad1Δiad2 (GRN7) | 0.04 ± 0.04 (6) | 2.5 ± 2.5 | 0.027 ± 0.010 (4) | 18.2 ± 6.8 |
| FB2Δiad1Δiad2 (GRN8) | 0.01 ± 0.02 (6) | 0.6 ± 1.3 | 0.033 ± 0.005 (4) | 22.3 ± 3.4 |
| GRN7Δnit1 (BH7) | 0.03 ± 0.00 (3) | 1.9 ± 0.0 | 0.042 ± 0.001 (4) | 28.4 ± 0.7 |
| GRN8Δnit1 (BH13) | 0.01 ± 0.02 (3) | 0.6 ± 1.2 | 0.035 ± 0.006 (4) | 23.6 ± 4.1 |
*
Strains were incubated in CM/Ara in the presence of 0.5 mm TAM or 5 mm Trp. Concentrations were calculated from peak areas resulting from HPLC analysis. The mean (± SD) values were calculated from three to six replicates (numbers in parentheses) of a single strain.