Table 2.
Influence of the Δtam1/Δtam2 deletions on the in vivo conversion of Trp to IAA and IPA.
| Strain | CM/Glc(*), (*,†) | CM/Ara(†), (*,†) | ||||
|---|---|---|---|---|---|---|
| IAA | IPA | IAA | ||||
| Mean ± SD (µm) | Mean ± SD (%) | Mean ± SD (µm) | Mean ± SD (%) | Mean ± SD (µm) | Mean ± SD (%) | |
| FB1 | 3.08 ± 0.37 | 100.0 ± 12.1 | 844.1 ± 91.5 | 100.0 ± 10.8 | 0.220 ± 0.032 | 100.0 ± 14.5 |
| FB2 | 2.52 ± 0.42 | 81.9 ± 13.7 | 243.6 ± 56.1 | 28.9 ± 6.6 | 0.239 ± 0.016 | 108.6 ± 7.5 |
| FB1Δiad1Δiad2 (GRN7) | 2.15 ± 0.33 | 69.8 ± 11.1 | 228.9 ± 24.0 | 27.1 ± 2.8 | 0.071 ± 0.004 | 32.3 ± 1.6 |
| FB2Δiad1Δiad2 (GRN8) | 1.85 ± 0.19 | 60.2 ± 6.2 | 296.9 ± 56.8 | 35.2 ± 6.7 | 0.063 ± 0.008 | 28.5 ± 3.8 |
| GRN7Δtam1‡ | 0.31 ± 0.07 | 10.0 ± 2.1 | 41.6 ± 13.0 | 4.9 ± 1.5 | 0.027 ± 0.008 | 12.3 ± 3.6 |
| GRN8Δtam1‡ | 0.34 ± 0.07 | 11.2 ± 2.2 | 46.7 ± 15.3 | 5.5 ± 1.8 | 0.033 ± 0.006 | 14.9 ± 2.6 |
| GRN7Δtam1Δtam2‡ | 0.22 ± 0.02 | 7.1 ± 0.7 | 20.2 ± 2.6 | 2.4 ± 0.3 | 0.029 ± 0.006 | 13.0 ± 2.6 |
| GRN8Δtam1Δtam2‡ | 0.17 ± 0.03 | 5.4 ± 0.9 | 14.4 ± 6.1 | 1.7 ± 0.7 | 0.030 ± 0.006 | 13.5 ± 2.8 |
| GRN7Δnit1 (BH7) | 2.29 ± 0.22 | 74.5 ± 7.1 | 488.5 ± 40.0 | 57.9 ± 4.7 | – | – |
| GRN8Δnit1 (BH13) | 1.41 ± 0.29 | 45.9 ± 9.3 | 172.4 ± 33.6 | 20.4 ± 4.0 | – | – |
(*)
Strains were incubated in CM/Glc (starting cell density of 2 × 107/mL) in the presence of 5 mm Trp .
(†)
Strains were incubated in CM/Ara in the presence of 5 mm Trp (†).
(*,†)
Concentrations were calculated from peak areas resulting from HPLC analysis. The mean (±SD) values were calculated from three replicates of a single strain (*,†).
(‡)
Two independent strains were investigated and all values averaged (‡).