Figure 2.

(A) Quantitative analysis and comparison of conidia produced by the wild‐type EV‐MIL31 (WT), the Alternaria alternata slt2 mutants (slt2‐D1, D2) and the genetically reverted strains (slt2‐D1/SLT2‐C6, C10) of A. alternata. Means (separation by Waller–Duncan k‐ratio t‐test, P ≤ 0.05) followed by the same letter are not significantly different. (B) Production of protoplasts over time by the A. alternata strains after exposure to cell wall‐degrading enzymes containing lyticase, driselase, β‐d‐glucanase and β‐glucuronidase. The release of protoplasts was determined with a haemocytometer by microscopy. Each column or point represents the mean number of conidia ± the standard error from two independent experiments, with at least three replicates.