Table 1.
Summary of 28 DR gene clones differentially expressed in both barley–Ptt and barley–Ptm incompatible interactions.
| Clone | Size (bp) | GenBank accession no. | Protein similarity | Sequence origin | BLASTx | Gene cluster | Chromosomal position and marker type | |
|---|---|---|---|---|---|---|---|---|
| Accession no. of similar sequence | Similarity | |||||||
| Carbohydrate metabolism | ||||||||
| HvPtr6 | 203 | EY965255 | Sugar transporter | O. sativa | ABF94465 | 1e‐40 (73%, 115aa) | 2 | not mapped |
| HvPtr11 | 424 | EY965260 | Putative neutral/alkaline invertase | O. sativa | BAD33266 | 6e‐48 (94%, 97aa) | 3 | 6H, SSR |
| Detoxification | ||||||||
| HvPtr7 | 121 | EY965256 | Major facilitator superfamily protein | O. sativa | EAZ19564 | 8e‐08 (80%, 45aa) | 2 | 7H, RFLP |
| HvPtr8 | 393 | EY965257 | Putative sterol delta‐7 reductase | A. thaliana | BAD19845 | 8e‐60 (90%, 130aa) | 2 | 3H, CAPS |
| HvPtr12 | 290 | EY965261 | Mitochondrial aldehyde dehydrogenase | H. vulgare | BAB62757 | 4e‐10 (72%, 50aa) | 3 | 1H, RFLP |
| HvPtr15 | 249 | EY965264 | Aldehyde dehydrogenase | O. sativa | AAG43027 | 3e‐29 (95%, 82aa) | 3 | 5H, PCR |
| HvPtr17 | 278 | EY965266 | Putative cytochrome P450 | L. rigidum | AAK38091 | 8e‐45 (91%, 92aa) | 3 | 7H, PCR |
| HvPtr23 | 275 | EY965272 | MATE efflux protein family | O. sativa | BAC83974 | 6e‐07 (50%, 63aa) | 5 | not mapped |
| HvPtr27 | 176 | EY965276 | Putative flavin mono‐oxygenase | O. sativa | EAY93143 | 2e‐22 (82%, 58aa) | 6 | 7H, RFLP |
| Signal transduction | ||||||||
| HvPtr2 | 366 | EY965251 | Putative receptor‐like protein kinase | O. sativa | AAP68881 | 4e‐06 (69%, 42aa) | 1 | 5H, RFLP |
| HvPtr3 | 392 | EY965252 | 14‐3‐3‐like protein A | H. vulgare | P29305 | 2e‐52 (100%, 103aa) | 1 | 4H, CAPS |
| HvPtr5 | 452 | EY965254 | Putative receptor‐like protein kinase | O. sativa | CAH67715 | 1e‐41 (87%, 93aa) | 2 | 2H, RFLP |
| HvPtr9 | 319 | EY965258 | Putative B‐box‐type zinc finger protein | O. sativa | BAD46368 | 1e‐60 (77%, 148aa) | 2 | 5H, CAPS |
| HvPtr14 | 159 | EY965263 | Putative ser/thr protein kinase | O. sativa | BAC79157 | 4e‐17 (83%, 48aa) | 3 | 5H, CAPS |
| HvPtr18 | 156 | EY965267 | Leucine‐rich repeat protein family | O. sativa | AAV31268 | 4e‐36 (73%, 109aa) | 4 | 1H, CAPS |
| HvPtr19 | 152 | EY965268 | Casein kinase I‐like | O. sativa | BAB92346 | 2e‐20 (96%, 50aa) | 4 | 3H, SSR |
| Calcium signal perception | ||||||||
| HvPtr1 | 111 | EY965250 | Ser/thr protein kinase, NPH1‐1 | A. sativa | AAC05083 | 5e‐48 (89%, 104aa) | 1 | not mapped |
| HvPtr10 | 499 | EY965259 | Putative calcineurin B‐like protein | O. sativa | AAL31965 | 5e‐22 (90%, 53aa) | 2 | 5H, CAPS |
| HvPtr24 | 260 | EY965273 | Putative copine I | O. sativa | BAD35622 | 5e‐22 (93%, 49aa) | 5 | 7H, PCR |
| Stress or defence response | ||||||||
| HvPtr4 | 154 | EY965253 | Chitinase | O. sativa | BAA23809 | 4e‐64 (75%, 172aa) | 2 | 3H, RFLP |
| HvPtr25 | 106 | EY965274 | Putative proteinase inhibitor | T. aestivum | AAS49905 | 5e‐43 (100%, 90aa) | 5 | not mapped |
| HvPtr26 | 217 | EY965275 | Pathogenesis‐related protein 1 | H. vulgare | CAA79703 | 9e‐17 (100%, 39aa) | 6 | not mapped |
| Miscellaneous | ||||||||
| HvPtr13 | 237 | EY965262 | Alpha‐galactosidase | H. vulgare | CAA74160 | 1e‐36 (97%, 78aa) | 3 | not mapped |
| HvPtr16 | 166 | EY965265 | CBS domain containing protein | O. sativa | EAY93850 | 3e‐22 (90%, 55aa) | 3 | 2H, SSR |
| HvPtr20 | 176 | EY965269 | Putative threonine aldolase | O. sativa | EAY94851 | 1e‐19 (82%, 58aa) | 5 | 2H, CAPS |
| HvPtr21 | 333 | EY965270 | Glutamate dehydrogenase | O. sativa | BAE48298 | 5e‐28 (90%, 63aa) | 5 | 2H, RFLP |
| HvPtr22 | 180 | EY965271 | Putative ABA‐responsive protein | O. sativa | BAD25079 | 8e‐59 (76%, 139aa) | 5 | not mapped |
| HvPtr28 | 413 | EY965277 | Putative iron/ascorbate‐dependent oxidoreductase | O. sativa | EAZ36021 | 4e‐27 (59%, 99aa) | 7 | 6H, CAPS |
Note: protein similarities for clones in bold type were derived from the best barley EST match. aa, amino acids.