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. 2019 Feb 20;4:37. [Version 1] doi: 10.12688/wellcomeopenres.15119.1

Table 1. ALT-R XT CRISPR RNA (crRNA) and single-stranded oligo DNA (ssODN) sequences for CD46 targeting.

Description Sequence
crRNA-CD46 5’-AAATGTTGGTGGCTCCTCAC-3’
ssODN-
CD46-HiBiT
5’-GTACTACCTGCTGCCAGACC
ACAGTCCATGGCTGATGAA
AGTGATATCAGTACTTCATC
TTCATGTTCCTATTCTCTTAT
CCCTAGATGCCGTGAGCGGC
TGGCGGCTGTTCAAGAAGAT
TAGCggaggtggaTGTGAGGA
GCCACCAACATTTGAAGCTA
TGGAGCTCATTGGTAAACC
AAAACCCTACTATGAGATTG-3’
ssODN-
CD46-V5
5’-GTACTACCTGCTGCCAGACC
ACAGTCCATGGCTGATGAA
AGTGATATCAGTACTTCATC
TTCATGTTCCTATTCTCTTAT
CCCTAGATGCCGGTAAGCCTA
TCCCTAACCCTCTCCTCGGTC
TCGATTCTACGggaggtggaTG
TGAGGAGCCACCAACATTTGAA
GCTATGGAGCTCATTGGTAAAC
CAAAACCCTACT-3’