Table 1. ALT-R XT CRISPR RNA (crRNA) and single-stranded oligo DNA (ssODN) sequences for CD46 targeting.
Description | Sequence |
---|---|
crRNA-CD46 | 5’-AAATGTTGGTGGCTCCTCAC-3’ |
ssODN-
CD46-HiBiT |
5’-GTACTACCTGCTGCCAGACC
ACAGTCCATGGCTGATGAA AGTGATATCAGTACTTCATC TTCATGTTCCTATTCTCTTAT CCCTAGATGCCGTGAGCGGC TGGCGGCTGTTCAAGAAGAT TAGCggaggtggaTGTGAGGA GCCACCAACATTTGAAGCTA TGGAGCTCATTGGTAAACC AAAACCCTACTATGAGATTG-3’ |
ssODN-
CD46-V5 |
5’-GTACTACCTGCTGCCAGACC
ACAGTCCATGGCTGATGAA AGTGATATCAGTACTTCATC TTCATGTTCCTATTCTCTTAT CCCTAGATGCCGGTAAGCCTA TCCCTAACCCTCTCCTCGGTC TCGATTCTACGggaggtggaTG TGAGGAGCCACCAACATTTGAA GCTATGGAGCTCATTGGTAAAC CAAAACCCTACT-3’ |