Figure 2.
FLS2 is not required for Arabidopsis resistance to Xanthomonas campestris pv. campestris strain 8004 (Xcc 8004). (A) Callose deposition in wild‐type (WT) Col‐0 and fls2 mutant leaves was determined at 24 h following inoculation of Xcc 8004 at 2 × 107 colony‐forming units (CFU)/mL. Average numbers of callose deposits per microscopic field of 0.1 mm2 are shown below each photograph. ‘±’ represents the standard deviations from three leaves of each plant, and similar phenotypes were observed in three independent experiments. (B) Xcc 8004 induces chlorosis in both Col‐0 and fls2 mutant. Symptoms were photographed 5 days after inoculation of Xcc 8004 at 1 × 107 CFU/mL. (C) Bacterial growth in Col‐0 and fls2 mutant. Five‐week‐old Arabidopsis plants were inoculated by Xcc 8004 at 5 × 105 CFU/mL, and the bacterial population in the leaf was determined at the indicated times. Each data point represents three replicates. Error bars indicate standard deviations. The experiments were repeated twice with similar results. (D) Alignment of FliC sequences corresponding to flg22 in Xcc B305 (eliciting), Xcc B186 (noneliciting) and Xcc 8004.