Table 2.
Relative up‐ or down‐regulation of selected defence‐related genes in inoculated compared with mock‐inoculated CR plants during the seedling, tillering and two‐node stages of wheat development.
| dpi | Seedling stage | Tillering stage | Two‐node stage | ||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 0 | 1 | 1.5 | 2 | 2.5 | 3 | 4 | 5 | 6 | 0 | 1 | 2 | 3 | 4 | 4.5 | 5 | 5.5 | 6 | 0 | 1 | 2 | 3 | 4 | 4.5 | 5 | 5.5 | 6 | |
| SN15 | = | ‐ | ++ | = | = | = | ++ | = | = | = | ‐‐‐ | ‐ | + | = | = | = | = | = | NA | NA | NA | NA | NA | NA | NA | NA | NA |
| SN18 | = | = | = | = | = | = | = | = | = | = | ‐ | ‐‐ | + | = | = | = | ‐ | = | = | = | = | ‐‐ | ‐‐ | = | ++ | = | ‐ |
| SN33 | = | = | ‐ | = | = | + | = | = | = | = | = | = | = | + | + | = | + | + | = | ‐ | = | + | ‐ | ‐ | ++ | = | = |
| SN41 | = | = | = | = | = | = | +++ | + | = | = | ‐‐ | = | ++ | = | ‐ | = | + | = | = | = | = | = | ++ | = | + | ++ | ++ |
| SN42 | = | = | = | + | = | = | = | = | = | = | ‐‐ | ‐ | ++ | ‐ | ‐ | = | = | = | = | ‐‐ | ‐ | ++ | ‐ | ‐ | = | = | = |
| SN64 | = | = | = | = | = | = | ++ | + | = | = | ‐ | = | + | ‐‐ | = | = | ++ | ‐‐ | = | ‐ | = | = | ‐‐ | = | = | = | ‐ |
| SN81 | = | = | = | = | = | = | ++ | = | = | = | ‐ | = | = | = | = | + | + | ‐ | = | + | = | = | ++ | = | = | + | + |
The expression data of SN15 are not available (NA) for the two‐node stage. The expression of defence‐related genes was normalized by expression of the reference control (wheat 18S gene) and the ΔΔCT method of relative gene quantification was used to calculate the expression level of CR inoculated relative to CR mock‐inoculated. A gene was considered as differentially regulated if the relative difference between the two treatments was higher than 2. Key: = no difference in expression between inoculated and mock‐inoculated leaves (P > 0.001);
‐ gene lightly down‐regulated in inoculated leaves (fold difference < 2 and P ≤ 0.001);
‐‐ gene down‐regulated in inoculated leaves (2 < fold difference < 5 and P ≤ 0.001);
‐‐‐ gene highly down‐regulated in inoculated leaves (fold difference > 5 and P ≤ 0.001);
+ gene lightly up‐regulated in inoculated leaves (fold difference < 2 and P ≤ 0.001);
++ gene up‐regulated in inoculated leaves (2 < fold difference < 5 and P ≤ 0.001);
+++ gene highly up‐regulated in inoculated leaves (fold difference > 5 and P ≤ 0.001).