Table 4.

Oligonucleotide primers used to measure relative expression of different TDFs using real‐time PCR.

TDF	Foward primer (5′–3′)	Reverse primer (5′–3′)	T m value (°C)
18S	AGGAGTTCCAGCACATCCTTC	CCCTCTTGTTCATGTCGATGT	60
SN15	AAGAATGAGTGGGGTTGGTG	GAAGGGAATCTGCAAATGGA	60
SN18	AAAGCTGCTCCCTCCATACC	CTGCGTACCAATTCACAGAAA	60
SN33	CTCCTTGTCCATCTTATCAGAAAGC	GGGCATTTGAGATTGCTAGTTCAC	60
SN41	GCCCGCTTCACAATCTTTGG	TGCCAGTGATTGCCTTGGT	60
SN42	GATGGTCAGTAGGCGGTAGC	ACTGTGGCTGCTTGCTTCTT	60
SN64	TGAAGTGTTTGTCAGATGATCCAAT	CTGTTTTCCCATCGCCAATTT	60
SN81	ATACCGTTTTCGCGGTTATG	CTTGAGGAGTCGACGGAGAT	60