Figure 2.
The tzs‐fs mutant shows lower tumorigenesis and transient transformation efficiency in Arabidopsis roots. Root segments from 3–4‐week‐old Arabidopsis plants were infected with the wild‐type NT1RE(pJK270), tzs‐fs mutant and tzs‐complemented Agrobacterium tumefaciens strains at two concentrations [108 and 107 colony‐forming units (cfu)/mL] for both tumorigenesis (A) and transient transformation (B) assays. The transformation efficiency of root segments infected with the wild‐type strain NT1RE(pJK270), with approximately 40%–70% of roots forming tumours or β‐glucuronidase (GUS) activity in each experiment, was set at 100% and that of the mutant is shown relative to that of the wild‐type strain. Mean values of virulence from at least three independent experiments are shown with standard errors. (C) Representative plates of Arabidopsis roots with tumours infected at a bacterial concentration of 108 cfu/mL.