Figure 3.
Cytokinin supplementation during infection increases the transient transformation efficiencies of strains lacking Tzs. Arabidopsis root segments were infected with a bacterial concentration of 107 colony‐forming units (cfu)/mL of various Agrobacterium tumefaciens strains, the wild‐type (NT1RE[pJK270]), the tzs‐fs mutant, A208, LBA4404 and EHA101, on Murashige and Skoog (MS) medium with or without different kinetin concentrations for 2 days. Seven days after infection, root segments were stained with 5‐bromo‐4‐chloro‐3‐indolyl‐β‐d‐glucuronic acid (X‐gluc) staining solution. Mean values of the transformation frequencies from at least three independent experiments are shown with standard errors. An asterisk above each mean represents a statistically significant difference between the transformation frequencies of the wild‐type and various tested strains in the absence of exogenous cytokinins during infection based on analysis of variance (anova) and pairwise Student's t‐test. P < 0.05 for all significant differences between strains. The letters ‘a’, ‘b’ and ‘c’ above each mean represent statistically significant differences with and without exogenous cytokinins using the same strain during infection based on anova and pairwise Student's t‐test. GUS, β‐glucuronidase.