. 2010 May 19;11(5):677–690. doi: 10.1111/j.1364-3703.2010.00637.x

Table 2.

Bacterial strains and plasmids.

Strains/plasmids	Relevant characteristics	References/sources
Strains Agrobacterium tumefaciens
C58	Wild‐type virulent strain containing nopaline‐type Ti plasmid pTiC58	Lin and Kado, 1977
A136	C58 cured of its Ti plasmid pTiC58	Watson et al., 1975
A208	A136 containing nopaline‐type Ti plasmid pTiT37	Sciaky et al., 1978
LBA4404	Ach5 strain containing a disarmed octopine‐type Ti plasmid pAL4404	Hoekema et al., 1983
EHA101	A136 strain containing a disarmed agropine‐type Ti plasmid pEHA105	Hood et al., 1986
NT1RE	Rm^R Em^R, C58 cured of its pTiC58	Watson et al., 1975
NT1RE(pJK270)	Rm^R Em^R, Km^R/Nm^R, pJK270 is pTiC58Tra^C with Tn5 insertion in T‐DNA region without affecting virulence	Kao et al., 1982
NT1RE(pJK270tzs‐fs)	Rm^R Em^R, Km^R/Nm^R, pJK270 containing tzs frameshift mutation	This study
Escherichia coli
DH5α	Host for DNA cloning	Hanahan, 1983
S‐17	Host for conjugation	Simon et al., 1983
Plasmids
pGEMT‐Easy	Ap^R, TA cloning vector	Promega, Madison, WI, USA
pUC19	Ap^R, cloning vector	US Biochemical, Cleveland, OH, USA
pBluescript SK+	Ap^R, cloning vector for blue/white screening	Stratagene, Cedar Creek, TX, USA
pCAMBIA 2201	Cm^R, a binary vector for plant transformation	Hajdukiewicz et al., 1994, Hiei et al., 1994
pCAMBIA 2201‐Gm	Cm^R, Gm^R, a binary vector for plant transformation	This study
pJQ200KS	Plasmid containing Gm^R and sacB gene for selection of double crossover	Quandt and Hynes, 1993
pJQ200KS‐tzs‐fs	Gm^R, pJQ200KS carrying tzs frameshift mutation	This study
pRU1064	Ap^R, Tc^R, a stable broad host range vector with IncP replicon	Karunakaran et al., 2005
pIncP‐Tzs	Ap^R, Tc^R, a pRU1064‐derived IncP plasmid carrying the promoter region and full length of tzs gene	This study
pEML771	Ap^R, Tc^R, an IncP plasmid with deletion of tzs open reading frame from pIncP‐Tzs	This study

Ap^R, Cm^R, Em^R, Gm^R, Km^R, Nm^R, Rm^R, Sp^R and Tc^R, resistant to ampicillin, chloramphenicol, erythromycin, gentamycin, kanamycin, neomycin, rifampicin, spectinomycin and tetracycline, respectively.