Figure 2.

Virulence of (leaf symptoms caused by) Xanthomonas campestris pv. campestris (Xcc) B305 on Nicotiana benthamiana is greatly attenuated by the loss of the hrcC gene despite sustained growth in planta. (A) Necrosis caused by Xcc B305 strains 5 days after syringe infiltration of the same strain onto N. benthamiana leaf. No tissue collapse was evident until at least 3 days after inoculation. WT, wild‐type B305; ΔhrcC, B305ΔhrcC; ΔhrcC‐comp, B305ΔhrcC carrying a plasmid with wild‐type hrcC locus under the control of the native promoter. (B) Xcc populations in syringe‐infiltrated N. benthamiana leaves. (C) Xcc B305 populations in syringe‐infiltrated N. benthamiana leaves; dpi, days post‐inoculation. (B, C) Data are mean ± standard error; bar fill and x‐axis, or line and symbol type, denote bacterial genotypes as described in (A). *Significant difference from the wild‐type version of the same strain (t‐test, P < 0.05). cfu, colony‐forming units.