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. 2009 Dec 15;11(2):245–254. doi: 10.1111/j.1364-3703.2009.00601.x

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© 2009 The Authors

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The hypersensitive response of Nicotiana benthamiana to V2 expression. (A) A N. benthamiana plant infected with PaV2/PVX showing cell death in the agroinfiltrated area (indicated by the arrows) as well as on systemic leaves. (B) A N. benthamiana plant infected with the PVX vector. The damage caused by inoculation is indicated by the arrow, but there is no cell death in the surrounding inoculated tissue. (C) A N. benthamiana plant infected with PaV2^AS/PVX. The inoculated leaf is indicated by an arrow. (D) A N. benthamiana leaf inoculated with PaV2/35s showing cell death in the inoculated area. (E) A N. benthamiana plant agroinfiltrated with PaV2/PVXMD (the arrow indicates necrosis in the inoculated area). (F) A N. benthamiana plant infected with KoV2/PVX showing cell death in the agroinfiltrated areas (indicated by the arrows). Nicotiana tabacum leaves inoculated with PaV2/PVX (G) and PVX (H), and treated with trypan blue. After clearing with chloral hydrate, cells in the PaV2/PVX‐inoculated leaf retain the dye, confirming cell death.