Table 1.

Features of gene constructs and the results of their inoculation into Nicotiana benthamiana.

Gene construct*	Insert size (bp)	Deletions (no. of amino acids) [amino acid coordinates of the deletion]	No of symptomatic plants/no. of plants inoculated†	Hypersensitive response
PaV2/PVX	453	—	10/10	Yes
PaV2AS/PVX	453	—	5/5	No
PaV2dmN32/PVX	357	32, N‐terminal [1–32]	8/10	Yes
PaV2dmN60/PVX	273	60, N‐terminal [1–60]	8/10	Yes
PaV2dmC20/PVX	393	20, C‐terminal [130–149]	6/8	Yes
PaV2dmC40/PVX	333	40, C‐terminal [111–149]	5/7	Yes‡
PaV2dmC50/PVX	303	50, C‐terminal [101–149]	7/10	Yes§
PaV2dmC60/PVX	273	60, C‐terminal [91–149]	7/10	No
PaV2dmC80/PVX	213	80, C‐terminal [71–149]	6/10	No
PaV2dmC119/PVX	96	119, C‐terminal [30–149]	8/10	No
KoV2/PVX	351	—	5/5	Yes

^* Constructs in Potato virus X (PVX) are indicated as either the V2 gene of Papaya leaf curl virus (PaLCuV) (PaV2) or Cotton leaf curl Kokhran virus (CLCuKoV) (KoV2) or deletion mutants (dm) of the PaLCuV V2 gene with deletions at either the N‐terminal (N) or C‐terminal (C) ends. A PVX construct with the PaLCuV V2 gene inserted in the anti‐sense orientation is indicated by AS.

^† Numbers of plants showing symptoms of PVX infection. Combined results of three independent experiments.

^‡ Delayed necrotic response.

^§ Necrosis only in the agroinfiltrated area.