Table 2.
Primers used for the amplification of the V2 and C2 genes of Papaya leaf curl virus (PaLCuV), Cotton leaf curl Kokhran virus (CLCuKoV) and Cotton leaf curl Multan virus (CLCuMV).
| Construct | Primer | Orientation | Sequence* |
|---|---|---|---|
| PaV2/PVX | PaV2 F | Virion | AGATCGATATGACATATGGACCAATCAG |
| PaV2 R | Complementary | AGGTCGACCTAGGAACATCTGGACTTCTGT | |
| PaV2AS/PVX | PaV2 ASF | Complementary | AGGTCGACATGACATATGGACCAATCAG |
| PaV2 ASR | Virion | AGATCGATCTAGGAACATCTGGACTTCTGT | |
| PaV2dmN32/PVX† | PaV2 N32 | Virion | AGATCGATATGTGGGATCCGTTATTGAACG |
| PaV2dmN60/PVX† | PaV2 N60 | Virion | AGATCGATATGTATAATCATTTCCACG |
| PaV2dmC20/PVX‡ | PaV2 C20 | Complementary | AGGTCGACCTACATGCTTTTGCTTTGGTGACGC |
| PaV2dmC40/PVX‡ | PaV2 C40 | Complementary | AGGTCGACCTAGGGCTGTCGAAGTTGAG |
| PaV2dmC50/PVX‡ | PaV2 C50 | Complementary | AGGTCGACCTATTCGAAGCGGGCGTGGAAATG |
| PaV2dmC60/PVX‡ | PaV2 C60 | Complementary | AGGTCGACCTAGCTGGTCGCTTCGACATAAT |
| PaV2dmC80/PVX‡ | PaV2 C80 | Complementary | AGGTCGACCTATCCCAGAGTATCTGGGGAATAC |
| PaV2dmC119/PVX‡ | PaV2 C119 | Complementary | AGGTCGACCTAGGTGAATGGAAAACACA |
| PaC2/35S | PaC2F | Complementary | AGATCGATATGCGATCTTCGTCACCCTCG |
| PaC2R | Virion | AGGTCGACTTAAAGACCCTTAAGAAACGA | |
| KoV2/PVX | CLCuKoV2F | Virion | GTTATCGATATGTGGGATCCACTGTTAAAT |
| CLCuKoV2R | Complementary | TCAGTCGACCTAGGAACATCTGGACTTCTG | |
| MuC2/35S | CLCuMVC2F | Complementary | GCCTCCCGGGATGCAATCTTCATCACTCTC |
| CLCuMVC2R | Virion | AATAGAATTCTTAATTGAAATTACACCGAG |
*
Restriction endonuclease recognition sequences used to clone the polymerase chain reaction (PCR) fragments are given in italic.
†
Amplification of the gene for these constructs used PaV2 R as the complementary‐sense primer.
‡
Amplification of the gene for these constructs used PaV2 F as the virion‐sense primer.