Table 3. Details of mice used for chimera contribution assay.
Zmym2 KD or OE iPSCs were injected into E4.5 C57Bl6 host blastocysts. The resulting embryos were implanted into pseudopregnant females and the pups analysed for iPS contribution by coat colour. M:male, F:female, GLT: germline transmission.
| Contribution | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Date | Clone
name |
No. of
Females |
Strain | No. of
embryos |
No.
injected |
No.
transferred |
No.
recipients |
No.
pregnant |
No.
born |
No. of
chimeras |
M | F | high | medium | Low | GLT |
| 13/05/2013 | Zmym2
KD |
12 | C57Bl6 | 74 | 70 | 70 | 5x25 | 2 | 17 | 5 | 3 | 2 | 0 | 5 | 0 | Yes |
| 14/06/2013 | Zmym2
OE |
9 | C57Bl6 | 21 | 20 | 20 | 2x25 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | N/A |