Figure 3.

Histochemical staining for putative growth factors in gallbladders inoculated with C. jejuni. (A) The mucosal surface and deep invaginations are lined with moderate amounts of acid mucin (deep blue staining) and there are pools of acid mucin within the lumen of invaginations at 2 h post-inoculation. Alcian blue (pH 2.5). (B) At 24 h post-inoculation acid mucin staining is largely absent in the necrotic surface mucosa but is still marked within the cells and the lumen of deep mucosal invaginations. Alcian blue (pH 2.5). (C) The apical cytoplasm of mucosal epithelial cells and the lumen of mucosal invaginations contain large amounts of neutral mucin (purple staining) 2 h post-inoculation. PAS staining. (D) Neutral mucin staining is also marked within submucosal glands. PAS staining. (E) 2 h post-inoculation the mucosal surface and mucosal invaginations are lined with large amounts of L-fucose (brown staining). Lectin staining for L-fucose. (F) The deep mucosal invaginations and submucosal glands also contain large amounts of L-fucose 2 h post-inoculation. Lectin staining for L-fucose.