Table 1.
Summary of histologic changes associated with direct inoculation of the ovine gallbladder with C. jejuni.
| Mucosal changes | Muscularis changes | Serosal changes | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sheep ID | Ulceration | Gland abscesses | Eosinophils within the mucosa and lamina propria | Mott cells within the mucosa and lamina propria | Lymphoid nodules | Neutrophilic infiltration | Vascular congestion/lymphatic dilation | Edema | Neutrophilic infiltration | |
| 2 h | 2.1a | 0 | 3 | 2 | 1 | 1 | 2 | 1 | 3 | 1 |
| 2.2b | 0 | 1 | 2 | 1 | 0 | 2 | 1 | 2 | 2 | |
| 2.3 | 0 | 3 | 0 | 3 | 1 | 1 | 3 | 2 | 0 | |
| 2.4b, c | 0 | 1 | 2 | 2 | 2 | 2 | 1 | 1 | 0 | |
| 24 h | 24.1b | 1 | 2 | 1 | 0 | 1 | 3 | 3 | 2 | 3 |
| 24.2b | 3 | 0 | 2 | 3 | 0 | 3 | 3 | 3 | 1 | |
| 24.3 | Unable to score due to 2-3 cm of transmural necrosis, demarcated by a band of degenerate neutrophils and cellular debris | |||||||||
| 24.4c | 0 | 2 | 1 | 1 | 2 | 2 | 1 | 1 | 2 | |
| Controld | 1 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 0 | 0 |
| 2b | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | |
0 = none/no lesion of that type present; 1 = mild lesion present; 2 = moderate lesion present; 3 = severe lesion present.
a
pre-inoculation bile positive for growth of C. jejuni.
b
sample processed for immunohistochemistry.
c
sample processed for SEM.
d
gallbladder samples taken from uninoculated sheep.