Table 1.
Effect of transient overexpression and TIGS of Prx genes on basal resistance of barley and wheat.
| Construct* | Relative SI (% of empty vector control)† | |||
|---|---|---|---|---|
| Overexpression | TIGS | |||
| Barley G.Pr.‡ | Wheat | Barley G.Pr. | Barley Ingrid | |
| pGY1_HvPrx08 | 70 ± 11 | n.a. | — | — |
| (OEx HvPrx08) | (P = 0.0391) | |||
| pIPKTA9_HvPrx40 | 83 ± 4 | 45 ± 3 | — | — |
| (OEx HvPrx40) | (P = 0.0008) | (P < 0.0001) | ||
| pWIR3§ | 54 ± 4 | 27 ± 2 | — | — |
| (OEx TaPrx103) | (P < 0.0001) | (P < 0.0001) | ||
| pHvPrx07hairpin¶ | — | — | 116 ± 56 | 124 ± 45 |
| (TIGS HvPrx07) | (P = 0.7872) | (P = 0.5527) | ||
| pIPKTA30N_HvPrx40 | — | — | 184 ± 28 | 112 ± 10 |
| (TIGS HvPrx40) | (P = 0.0105) | (P = 0.2547) | ||
| pIPKTA34** | — | — | 14 ± 4 | 11 ± 2 |
| (TIGS HvMlo) | (P = 0.0001) | (P < 0.0001) | ||
*
All constructs contain the CamV 35S promoter and terminator. See Fig. 3.
†
SI, susceptibility index = number of GUS‐positive cells containing at least one haustorium/total number of GUS‐positive cells.
‡
G.Pr, barley cv. Golden Promise.
§
Described by Schweizer et al. (1999).
¶
Described by Christensen et al. (2004).
**
Described by Douchkov et al. (2005).