Table 2.

Specific silencing of a HvPrx40:GFP fusion gene by the co‐bombarded RNAi construct that targets HvPrx40.

Co‐bombardment	GFP cells*	GFP/GUS†
pUbiGUS+pGFP+pIPKTA30N	951.5 ± 89.5	2.30 ± 0.18 (100)
pUbiGUS+pGFP+pIPKTA30N_HvPrx40	812.0 ± 144.0	2.04 ± 0.25 (89)
pUbiGUS+pGFP+pIPKTA30N_HO14J07‡	682.5 ± 30.5	1.89 ± 0.19 (82)
pUbiGUS+pIPKTA40_HvPrx40deltaSP+pIPKTA30N	37.0 ± 2.0	0.10 ± 0.01 (100)
pUbiGUS+pIPKTA40_HvPrx40deltaSP+pIPKTA30N_HvPrx40	16.3 ± 6.3	0.03 ± 0.01 (30)
pUbiGUS+pIPKTA40_HvPrx40deltaSP+pIPKTA30N_HO14J07	138.0 ± 10.5	0.35 ± 0.07 (359)

^* Number of GFP‐expressing cells per bombardment. Means ± range of two independent experiments.

^† Ratio of GFP‐expressing to GUS‐expressing cells. Mean ± range of two independent experiments. Values in parentheses indicate relative values in percentage of the corresponding empty RNAi‐vector control.

^‡ Control RNAi construct targeting a transaldolase gene (represented by EST clone HO14J07).