Table 3.

Hydrogen peroxide accumulation in cell‐wall appositions upon transient silencing of HvPrx40.

	DAB‐stained CWA (% of surrounding cells)*
	pIPKTA30N	pIPKTA30N_HvPrx40
Bombardment 1	81.9	99.1
Bombardment 2	97.0	87.0
Bombardment 3	109.9	76.8
Bombardment 4	110.3	86.7
Bombardment 5	97.1	117.1
Mean	99.2	93.3
SEM	5.2	6.9
p (one sample t‐test)	0.891	0.39

^* Local accumulation of hydrogen peroxide in cell‐wall appositions (CWA) beneath fungal penetration attempts was monitored by DAB staining. The percentage of DAB‐stained CWA of GUS‐positive, transformed cells was compared with four surrounding, attacked cells (north, east, south and west) that were not transformed. The analysis is based on a total of 119–124 GUS‐positive, attacked cells per construct.