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. 2007 Dec 18;9(2):237–250. doi: 10.1111/j.1364-3703.2007.00456.x

Figure 2.

Figure 2

Detection of Raspberry bushy dwarf virus (RBDV) by (A) reverse transcription polymerase chain reaction (RT‐PCR) and (B–D) reverse transcription loop‐mediated isothermal amplification (RT‐LAMP). Primers were designed according to the coat protein (CP) gene of RBDV. (A) RT‐PCR: lane P, positive control (cloned RBDV CP gene); lane N, negative control (virus‐free raspberry clone TTA‐508); lanes 1–5, RBDV‐infected plants of raspberry line Z13. (B) Amplification of RBDV cDNA detected by turbidity caused by the magnesium pyrophosphate precipitate. P and N as above; B, reaction without template; 1–3, RBDV‐infected plantlets of line Z13. (C) The products of RT‐LAMP obtained in B analysed by agarose gel electrophoresis. The samples are in the same order as in the test tubes in B above. (D) Indexing of raspberry plants for RBDV by RT‐LAMP: P and N as above; 1 and 2, RBDV‐free plantlets of line Z13 obtained by thermotherapy followed by cryotherapy.