Table 2.
List of bacterial strains and plasmids used in this study.
| Bacterium or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Xanthomonas. perforans | ||
| 91‐118 | Wild‐type, pathogenic to tomato, Rifr | Jones et al. (2004) |
| 91‐118hrp− | Same as 91‐118, hrp cluster mutated with NTG, complemented with hrp clone, Rifr | G. V. Minsavage |
| 91‐118ΔxopA X. axonopodis pv. citri | XopA‐RifR | Hert (2007) |
| Xac‐A 40 | Wild‐type, Asiatic strain, isolated in Argentina, Rifr | DPI |
| Xac‐A 306 | Wild‐type, Asiatic strain, isolated in Brazil, Rifr | DPI |
| Xac‐Aw 12879 | Wild‐type, Wellington strain, pathogenic to Key lime | DPI |
| Xac‐AwΔavrGf1 | Wellington strain, Ω cassette inserted into avrGf1 | This work |
| Xac‐AwΩ4 | Wellington strain, Ω cassette inserted into avrGf1, pthAw and avrTaw | This work |
| Xac‐A* 1974 | A* strain (Xc290) | (Sun et al., 2004; Vernier et al., 1998), DPI |
| Xac‐A* 1975 | A* strain (Xc280) | (Sun et al., 2004; Vernier et al., 1998), DPI |
| X. axonopodis pv.aurantifolii | ||
| Xaa‐B 1622 | B‐69, isolated in Argentina | DPI |
| Xaa‐C 5979 | Xc 70, isolated in Brazil | DPI |
| X. axonopodis pv. citrumelo | ||
| Xac‐E 1887 | Wild‐type | DPI |
| X. campestris pv. campestris | ||
| Xcc 8004 | Wild‐type, Rifr | Metz et al. (2005) |
| Escherichia coli | ||
| DH5α | F−recA hsdR17(rk‐mk+) Φ80dLacZ | Bethesda Research Laboratories, Bethesda, MD, USA |
| HB101 | F− recA | Maniatis et al. (1982) |
| Plasmids | ||
| pLAFR3 | Tra−Mob+, RK2 replicon, tetr | Staskawicz et al. (1987) |
| pLAFR6 | pLAFR1 with trp terminators | Bonas et al. (1989) |
| pUFR034 | Inc W, Kmr, Mob+, lacZα, Par+, cosmid | De Feyter et al. (1990) |
| pUFR051 | pLAFR3 derivative | De Feyter et al. (1990) |
| pBluescript II SK(+) | Phagemid, pUC derivative, Ampr | Stratagene (La Jolla, CA, USA) |
| pHOKmGus | Kmr Apr Tn3‐uidA fusion | Bonas et al. (1989) |
| pUC8Ω | Vector with Ω cassette | B. Staskawicz |
| PGEM®T Easy Vector | Multiple site cloning vector | Promega |
| pOK1 | Suicide vector, pKNG101 derivative, Smr/Sucs | Huguet et al. (1998) |
| pRK2073 | ColE1 replicon, TraRK+, Mob+, Specr | Figurski and Helinski (1979) |
| pAW5.2 | 5‐kb EcoRI‐KpnI fragment containing pthA w from X0053 cloned in pUFR047 that complements pustule formation in a pustule minus strain | Al‐Saadi et al. (2007) |
| pL799 | pLAFR3 with DNA fragment from Xac‐Aw that contains avrGf1 | This study |
| pL799‐1 | pL799 with Tn3‐Gus insert in avrGf1 | This study |
| pL799‐2 | 3.0‐kb fragment from pL799 in pLAFR6 | This study |
| pU799‐3 | 2.3‐kb fragment containing avrGf1 in pUFR034 | This study |
| pBs3.0 | pBluescript II KS with 3.0‐kb insert from pL799‐1 containing Tn3‐KmGus | This study |
| pL22 | XacAw pLAFR3 cosmid that contains hrpX, hrpG and hsp90Xo homologues | This study |
| pL22E | Xac‐Aw pLAFR3 clone containing hrpG | This study |
| p0346 | Xac‐Aw pUFR034 clone containing hrpG | This study |
| pL689 | Similar to pL22 | This study |
| pLXOPA | 91‐118 pLAFR3 cosmid that contains the xopA gene | This study |
| pL80 | XacAw pLAFR3 clone that contains avrBs3 homologue, designated avrTaw | This study |
| pL104 | Similar to pL80 | This study |
| pL115 | Similar to pL80 | This study |
| pL622 | Similar to pL80 | This study |
| pUFR80.1 | Subclone of pL80 containing only avrTaw homologue that does not complement pustule formation | This study |
| pLAT211 | pLAFR3 containing avrBs3‐2 | Bonas et al. (1991) |
DPI, Division of Plant Industry of the Florida Department of Agriculture and Consumer Services, Gainesville, FL, USA; NTG, N‐methyl‐N′‐nitro‐N‐nitrosoguanidine; B. Staskawicz, University of California, Berkeley, CA, USA.